View instructions for seeding suspension cells.Īgilent Seahorse XF p HS Mini Assays are performed in an Agilent Seahorse 96-well 24-well 8-well XF p HS Mini Cell Culture Microplate Miniplate in conjunction with an XFe96 Sensor Cartridge. Core Facilities with Seahorse XF Research Servicesīasic procedures for seeding adherent cells (typically performed the day before the XF p HS Mini assay)įor each density to be tested, seed as directed for adherent cells.
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Suspension cells require higher seeding density from 5 x 10 4 to 2 x 10 5 cells per well depending on cell type. Optimal cell seeding number varies by cell type, but is typically between 5 x 10 3 and 4 x 10 4 cells per well for adherent cells. The Seahorse XF Real-Time ATP rate assay Test Kit The Seahorse XFp Real-Time ATP rate assay Test Kit can be used to characterize the cell line/type of interest in a single assay. To effectively examine metabolic and bioenergetic function using your Agilent Seahorse Extracellular Flux Analyzer, Analyzer, it is essential to first characterize a specific cell type with respect to its metabolic activity under basal and maximal respiration (OCR) and extracellular acidification (ECAR). Support Services, Agreements & Trainingīasic procedures for choosing cell seeding densities.Shorten the time it takes to start seeing the full value of your instrument investment Instrument & equipment deinstallation, transportation, and reinstallationĬrossLab Connect services use laboratory data to improve control and decision-makingĪdvance lab operations with lab-wide services, asset management, relocation Learn essential lab skills and enhance your workflows Instrument/software qualifications, consulting, and data integrity validations
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